| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Marino, Olivia (11)
|
ADGUY |
34.59 |
34.19 |
-0.40 |
| 2 |
Armstrong, Chloe V (11)
|
ADDACC |
34.68 |
34.23 |
-0.45 |
| 3 |
Schager, Kierra G (12)
|
ADDACC |
35.28 |
34.62 |
-0.66 |
| 4 |
Smith, Dannah J (11)
|
ADDACC |
34.95 |
34.84 |
-0.11 |
| 5 |
Trautner, Magdalene Anne B (11)
|
ADSARY |
35.97 |
35.21 |
-0.76 |
| 6 |
Steeves, Katherine E (11)
|
ADKING |
34.79 |
35.79 |
1.00 |
| 7 |
Dunn, Kaylie A (11)
|
ADDACC |
39.27 |
37.34 |
-1.93 |
| 8 |
Schmidt, Christina M (11)
|
ADKING |
36.70 |
37.40 |
0.70 |
| 9 |
Krein, Alanna (11)
|
ADGLEN |
40.46 |
37.86 |
-2.60 |
| 10 |
Uveges, Darlina (12)
|
ADBETH |
37.16 |
38.07 |
0.91 |
| 11 |
Sohn, Inari (12)
|
ADGBY |
37.78 |
38.55 |
0.77 |
| 12 |
Johnson, Stephanie P (11)
|
ADSSCY |
39.96 |
38.66 |
-1.30 |
| 13 |
Wendt, Emma (12)
|
ADGUY |
36.24 |
38.80 |
2.56 |
| 14 |
Ahmad, Safa (11)
|
ADSSCY |
41.18 |
40.00 |
-1.18 |
| 15 |
Brown, Meredith D (11)
|
ADGBY |
39.29 |
40.37 |
1.08 |
| 16 |
Krein, Ava (11)
|
ADGLEN |
40.46 |
40.56 |
0.10 |
| 17 |
Johnson, Erin (11)
|
ADDACC |
40.99 |
42.49 |
1.50 |
| 18 |
Albee, Rowan (11)
|
ADDACC |
42.51 |
43.09 |
0.58 |
| 19 |
Swift, Raven K (12)
|
ADGLFY |
45.04 |
43.87 |
-1.17 |
| 20 |
Meredith, Paige C (11)
|
ADGBY |
39.70 |
44.19 |
4.49 |
| 21 |
Nihill, Emma (11)
|
ADGBY |
44.87 |
45.03 |
0.16 |
|
Carulli, Olivia E (11)
|
ADSARY |
34.84 |
NS |
- |
|
Durante, Skylar I (11)
|
ADGLFY |
37.24 |
NS |
- |